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Virologica Sinica, 17 (4) : 331-335, 2002
Research Article
Molecular Cloning and Expression of the Chitinase Gene from Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus in E.coli
Joint—lab of Invertebrate Virology,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071.China
The PCR product of the HaSNPV chitinase gene,which was without the N—terminal signal
peptide and the C-terminal endoplasmic reticulum location signal,was cloned into a prokaryotic expres—
sion vector pProEXHTb.After the induction of IPTG,the chitinase gene was successfully expressed in
Eschechia coli DH5a.The expression product shown a molecular weight of 60 kDa,and it covered
about 40% of the total protein in E .coli.W estern blot analysis using an antibody derived from AcM —
NPV ehitinase confirm ed the expression product was a homologue of baculoviral chitinase.
  Published online: 5 Nov 2002
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