Hello guest, Register  |  Sign In
Virologica Sinica, 17 (4) : 331-335, 2002
Research Article
Molecular Cloning and Expression of the Chitinase Gene from Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus in E.coli
Joint—lab of Invertebrate Virology,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071.China
 Correspondence:
(197.90KB)  
Abstract
The PCR product of the HaSNPV chitinase gene,which was without the N—terminal signal
peptide and the C-terminal endoplasmic reticulum location signal,was cloned into a prokaryotic expres—
sion vector pProEXHTb.After the induction of IPTG,the chitinase gene was successfully expressed in
Eschechia coli DH5a.The expression product shown a molecular weight of 60 kDa,and it covered
about 40% of the total protein in E .coli.W estern blot analysis using an antibody derived from AcM —
NPV ehitinase confirm ed the expression product was a homologue of baculoviral chitinase.
  Published online: 5 Nov 2002
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved