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Virologica Sinica, 17 (4) : 350-353, 2002
Research Article
Purification of a Antiviral Protein in Plearotus citrinopileatus and Its Activities against Tobacco mosaic virus and Hepatitis B virus
Institute of Plant Virology,Fujian Agriculture and Forestry University,Fuzhou 350002,China
A antiviral protein。YP46—46,was isolated and purified to homogeneity from the mushroom,
Plearotus citrinopileatus.by precipitation of 40% 一60% saturation of ammonium sulfate followed by
DEAE-sepharose FF ion—exchange column chromatography。 Sephacryl M S一200 High Resolution
molecular sieve chromatography.The protein has a molecular weight of about 27.4kD by SDS-PAGE.
The concentration of the protein was 0.24/~g/mL when the inhibition rate against Tobacco mosaic
irus was 50% .Using an assay system based on HepG2.2.2.1 5 cell line,YP46—46 was studied for its
inhibitory ability against Hepatitis B virus.The result shows that the concentration of 50% inhibition
of HBsAg was 0.08ttg/mL,but low effect on HBeAg.
  Published online: 5 Nov 2002
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