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Virologica Sinica, 17 (4) : 354-357, 2002
Research Article
The Study on BIV Activity in Human Cells M T -4
1.College ofLife Sciences,Nankai University,Tia, in 300071,China
2.Instituteof Virology, Chinese Academy of Preventive Medicine,BeOing 100052,China
Bovine immunodeficiency virus(BIV)is a kind of Lentivirus belonging tO Retrovirus,and
up tO now.there is no report about itS infection tO human.In order to check BIV S infection to human
cells,we transfected BIVl27 cDAN into human cells MT一4.By RT~PCR,we checked the transcription
of BIV S gag gene,and determined the expression of gag or gag-pol gene in M T一4 cell by IFA.Re—
verse Transcriptase(RT)assay showed the reverse transcriptase of BIVl27 had been expressed,while
cell infection experiment indicated that BIV couldn t replicate in MT一4 cells.
  Published online: 5 Nov 2002
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