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Virologica Sinica, 17 (4) : 358-361, 2002
Research Article
Amplification and Clone of VP2-4-3 Gene of Very Virulent Infectious Bursal disease virus
The Institute of Bio Technology ,School of Agriculture,Shanghai JiaoTong University,Shanghai,201101,China
 Correspondence:
(152.19KB)  
Abstract
The methods of reverse transcription,polymerase chain reaction(PCR)amplification,and
cloning of full—length VP2—4—3 gene of a very virulent infectious Bursal disease virus(vvlBDV)strain
SH95 were developed.The use of random primer and a reverse transcriptase lacking RNase—H activity
produced full—length coding region and non—coding region eDNA copies of the viral genomic segments.
The 3060 base—pairs(bp)of VP2—4—3 were amplified by long and accurate PCR in a single step,SUC—
cessfully cloned and sequenced revealing their identity of IBDV.
  Published online: 5 Nov 2002
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