Hello guest, Register  |  Sign In
Virologica Sinica, 17 (4) : 367-370, 2002
Research Article
New Methods for Determining Induction Rate of Prophage in Lysogenic Bacteria
College of Life Sciences,Wuhan University,Wuhan 430072,China
 Correspondence:
(157.78KB)  
Abstract
Two new methods,colony counting method and plate inducing method,were established for
determining ultraviolet induction rate of prophage in the present study.Culture of lysogenic bacteria
(λ)which was induced hy ultraviolet irradiation and cultivated in dark was directly spread on the
plate,and induction rate of prophage was calculated based on the CFUs(colony form ing units).The
plate with mixture of lysogenic bacteria and indicator strain was induced by ultraviolet irradiation.The
induction rate of prophage was calculated based on the PFUs(plaque form ing units).These two
methods not only can determ ine precisely induction frequency of prophage。but also are easy tO ma—
nipulate ,and they can save efforts and apparatus,they are also easy to repeat.Furthermore,the m ix—
ture of Cordyceps sinensis extract and lysogenic bacteria was irradiated with U V .The results demon—
strate that the new methods are practical and easy tO conduct,and that Cordyceps sinensis has a strong
protection effect against U V—irradiation.
  Published online: 5 Nov 2002
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved