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Virologica Sinica, 17 (4) : 371-373, 2002
Brief Reports
Serology Investigatio and PCR Diagnosis of Avian M yelocytomatosis
1.Institute ofAnimal Science and Animal Medicine,ShanDong Agriculture University,TaiAn 271018
2.Institute of Animal Science and Animal Medicine,Inner Mongolia Agriculture University,Huhhot 010018,China
3.Examination Croup of Inner Mongolia Veterinary Station,Huhhot 010020
4.The Center of Biology EngineeringofInner Mongolia University,Huhhot 010025,Chian
We investigated the infection of Avian leukosis virus subgroup J myelocytomatosis using
EI ISA method in Inner Mongolia Region.The result indicated that the meat—type flocks of three dif—
ferent farms infection rate'.of ALV—J were 28.12%(9/32)、9.38%(3/32)and 8.33%(10/12),respec—
tively,and all laying chickens infection rate were 0%(0/35,0/10).We chose 6 chickens of ALV—J an—
tibody positive and 13 chickens of ALV—J antibody negative to do PCR test.The result indicated that
6 chickens of ALV—J antibody positive were all PCR positive,and 4 chickens were PCR positive among
1 3 chickens of AI V—J antibody negative.The result indicated that there were ALV—J virus not only in
AI V—J antibody positive chickens but also in some AI V—J antibody negative chickens.
  Published online: 5 Nov 2002
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