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Virologica Sinica, 18 (1) : 27-30, 2003
Construction of Agrotis segetum granulosis virus cDNA Library
Detector ofGene Biotechnology Laboratory,Institute ofMicrobiology xiaj~ gAcademy ofAgricultural Science, Xinjiang Wulumuqi 830000,China
Abstract:A general cDNA library of Agrotis segetum granulosis virus(ASGV)which was from infected
A grotis segetum(As)l arva has been constructed.The first and second cDNA strands were synthesized by
AMV reverse transcriptase from mRNA which was isolated from total RNA of infected As larva. So the
general cDNA library includes AsGV’S and AS’S, Th en the library of AsGV included 108 1 positive
clones were screened by spot hybridization, Th e labeled AsGV genome DNA probe With DIG kit was
cut by restriction enzyme of EcoR I and Hind 11. Th e sequence of positnive clones accord with the
genome of AsGV,and there are 59 coding sequence which were amplified with synthetic primer of As—
GV genome according entirely with the genome of AsGV.
  Published online: 2 Feb 2003
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