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Virologica Sinica, 18 (1) : 68-71, 2003
Analysis of the Sequence of Variable Region in vp2 of Infectiousbursal disease virus Isolated from Diferent Bird Species
1.HuadongResearchInstitute ofMedicalBiotechnics,Nanjing210002,China
2.NationalLaboratoryforOncogenes andRelatedGenes,.Shanghai Cancer Institute,Shanghai 200032,China
3.Institute ofVeterinaryScience,JiangsuAcademyofAgricultural Sciences,Nanjing210014,China
4.Jiangsu Institute ofPoultry Science,Yangzhou 225003,China
Abstract:According to the published cDNA sequences of Infectious bursal disease virus(IBDV),a pair
of primers to variable region in VP2 were designed for cDNA synthesis and PCR amplification of four
IBDV isolated from different bird species,CH,DU,GE and SP.The nucleotide sequences determined
have been compared to each other.Th e four isolates are closely related,the homology of the four iso—
lates being 97% at the nucleotied level and 98% at the amino acid leve1.Th eir two hydrophilic regions
and one heptapeptide were identical at the amino acid leve1. Th e results revealed that the ducks,geese
and sparrows naturally infected with IBDV not only could become the carriers or reservoirs in transmis—
sion but may take certain effect on the variation of IBDV.
  Published online: 2 Feb 2003
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