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Virologica Sinica, 18 (1) : 79-81, 2003
Genetic Analysis of Simian virus 40 from Peripheral Blood and Kidney of M acaque M onkeys
Chinese Academy ofMed&d Science,Peking Union Med&d University,Institute ofMed&d Biology,Kunming 650118, China
In order to investigate the detection methods and gene structure of SV40 ST-ag and LT—ag—
C in peripheral blood mononuclear cells (PBMC) and kidney cells from different Macaque monkeys,
using PCR, 4 positive templates were obtained firstly, then 4 ST—ag gene fragments and 4 LT—ag—C
gene fragments were achieved and sequenced. These antigens coding region of each isolate contain a
number of nucleotide differences when compared to the reference strain SV40——776 and the laboratory
strain SV40一B.These resuhs indicated that:1)SV40 DNA isolated from the PBMC and kidney cells
showed essentially identical sequences for ST—ag gene and LT—ag—C gene. 21 the presence of viral
DNA in P—BMC suggests that virus may spread with in the host by hematogenous routes. 3) a new
SV40 strain predominates in our monkey population, it contains a specific nucleotide and amino acid
changes in LT-ag—C termini, TCCTCACAG polynucleotide is inserted among nt2784/2783, SO
SerSerGIn iS inserted after amino acid 678 residue.
  Published online: 2 Feb 2003
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