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Virologica Sinica, 18 (2) : 104-107, 2003
The Interaction of Viral Nucleocapsid Protein with GRP94 and HSP27 in Brains of Suckling M ice Experimentally Infected with Hantaan virus
Department of Pathology,the Fourth Military Medical University,Xi'an 710032,China
To study the association of heat-shock protein GRP94 an d HSP27 with Hantaan virus
nucleocapsid protein(HTNV—NP)during viral replication,new—bom mice less than 72h of age were
experimentally infected with Hantaan virus intracerebrally.Brains of mi ce at the 8th day post-infection
were prepared for tissue sections an d extracts.Hantaan virus infection,GRP94,HSP27 expression an d
their localization were demonstrated by immunohistochemi cal staining.The possible interaction
between HTNV—NP an d GRIX)4.HSP27 was an alyzed by ELISA,immunoprecipitation.HTNV
infection led to increased expression of GRIX)4 but not HSP27 at cytoplasma of neurons in brains of
infected suckling mice.colocalizationof GRIX)4 with HTNV—NP,an d association of both GRP94 and
HSP27 with HTNV—NP.Th e results provide the basis for further research of the roles of HSPs in the
viral infection and replication.
  Published online: 15 Apr 2003
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