About the Journal
Author Services
Reader Services
Friend Links
Global Visitors
Virologica Sinica, 18 (2) : 115-118, 2003
Contents
Detection of Human cytomegalovirus Infection by PCR-Hybridization-ELISA
The Virus Institute of Medical College of Wuhan University,Wuhan 430071，China，2．The First Railway Meddle School,Wuhan 430033，China
Correspondence: 
(141.49KB)
Abstract
A PCR—M PH—ELISA method detecting HCMV infection was established．The PCR products
of HCMV DNA were binting—labeled by a 5’-biotinlabeled primer through sample am plification．After
the labeled product was mixed with a 5’-digoxin—labeled probe，hybridization was carried out for 2
mi nutes at 90℃ and for 1 minute at 55℃ ．then the hybridized product was captured on microplate
wells coated with streptoavidin and detected with a peroxidase—labeled an tibody and TMB substrate．
The sensitivity of the method was 2．5×1 04 copies／mL
． and higher than that of conventiona1 PCR and
ELISA method．The results did not cross—react with that of HSV1，HSV2，RuV,EBV and ADV DNA．
Th e method has potentia1 use for qualitative an d quantitative detection of HCMV infection．
of HCMV DNA were binting—labeled by a 5’-biotinlabeled primer through sample am plification．After
the labeled product was mixed with a 5’-digoxin—labeled probe，hybridization was carried out for 2
mi nutes at 90℃ and for 1 minute at 55℃ ．then the hybridized product was captured on microplate
wells coated with streptoavidin and detected with a peroxidase—labeled an tibody and TMB substrate．
The sensitivity of the method was 2．5×1 04 copies／mL
． and higher than that of conventiona1 PCR and
ELISA method．The results did not cross—react with that of HSV1，HSV2，RuV,EBV and ADV DNA．
Th e method has potentia1 use for qualitative an d quantitative detection of HCMV infection．
Published online: 15 Apr 2003
