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Virologica Sinica, 18 (2) : 115-118, 2003
Detection of Human cytomegalovirus Infection by PCR-Hybridization-ELISA
The Virus Institute of Medical College of Wuhan University,Wuhan 430071,China,2.The First Railway Meddle School,Wuhan 430033,China
A PCR—M PH—ELISA method detecting HCMV infection was established.The PCR products
of HCMV DNA were binting—labeled by a 5’-biotinlabeled primer through sample am plification.After
the labeled product was mixed with a 5’-digoxin—labeled probe,hybridization was carried out for 2
mi nutes at 90℃ and for 1 minute at 55℃ .then the hybridized product was captured on microplate
wells coated with streptoavidin and detected with a peroxidase—labeled an tibody and TMB substrate.
The sensitivity of the method was 2.5×1 04 copies/mL
. and higher than that of conventiona1 PCR and
ELISA method.The results did not cross—react with that of HSV1,HSV2,RuV,EBV and ADV DNA.
Th e method has potentia1 use for qualitative an d quantitative detection of HCMV infection.
Key Words: PCR;  ELISA;  Human cytomegaloirus
  Published online: 15 Apr 2003
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