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Virologica Sinica, 18 (2) : 129-133, 2003
Cloning and Sequencing of the Capsid Protein Gene of Rabbit hemorrhagic disease virus Isolate W X84
Animal Science and Veterinary Medicine College Yangzhou University , Yangzhou,225009,China
2.Animal Science College Shandong Agricultural University,Taian,271018,China
Abstract:The capsid protein(VP60)gene of Rabbit hemorrhagic disease virus(RHDV)isolate WX84
was amplified by RT.PCR and cloned into pGEM R-T vector for sequence.Th e sequence an alysis
showed that the nucleotide sequence of the vp60 gene of WX84 was 1 740 bp an d encoding a protein of
580 amino acids.Sequence comparison with other published RHDV vp60 genes showed that the
homology of the nucleotide was between 98.2% and 99.0% .Th e result showed that vp60 gene was a
highly conserved gene.
  Published online: 15 Apr 2003
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