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Virologica Sinica, 18 (2) : 137-140, 2003
Amplification with Random PCR method and Researches for the DNA Fragment of the genome of Chicken emb~o lethal orphan virus
Key Laboratory of Animal Disease Diagnostic& Immunology,Ministry of Agriculture ofPeople’Republic of China, NanjingAgricultural University,Nanjing 210095,China
While observing Duck viral hepatitis seed virus in chicken embryonic allantochoroin liquid.
another symmetry nonenveloped icosahedral viron with a diam eter of 7O tO 80 am was found.In order
to investigation this contam inating virus,we selected four random primers to am plify this unknown
virus by RT—PCR method an d PCR method respectively,that resulted three DNA fragments,showing
99.5% ,99.6% an d 99.5% homologue respectively with the genome of Adenovirus type I,or Chicken
embryo orphan lethal virus(CELOV)during the sequencing analysis(one cycle reaction)for them.
This results suggested that Duck viral hepatitis seed virus was contam inated by CELOV
  Published online: 15 Apr 2003
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