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Virologica Sinica, 18 (2) : 164-168, 2003
Expression and purification of HCLV E2 protein A,D antigenic domains and its preliminary application*
I Key lab ofAnimal Diagnostic and Immunology,Ministry ofAgriculture,Nanjing Agricultural University,Nanfing 210095,China
2,Institute of Animal Husbandry and Medicine,Shanghai agricultural science academy,Shanghai 201 106.China 3,Anima l SciencP department ofAgricultural College ofNinxia University,Yinchuan 750105,China
Abstract:In this experiment the conditions for the expressing of HCLV E2 glycoprotein A and D
an tigenic domains in E coli were optimized.On this basis,the expressed protein was purified by
dissolving the inclusion body in Tris.HC1 with 6 mol/L guan dinine an d passed throh a His Bind affinity
chromatography.The product was collected an d th e concentrated recombinan t protein was quentified.
Th e recombinan t protein was then used to coat 96一well ELISA plates an d applied to detect the titers of
the serums from swine immunized with HCLV vaccine.
  Published online: 15 Apr 2003
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