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Virologica Sinica, 18 (3) : 246-250, 2003
Research Article
cDNA Clone and Sequence Analysis of Segment 4 of Dendrolimus punctatus cytoplasmic polyhedrosis virus
Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan430071,China
 Correspondence:
(248.07KB)  
Abstract
Abstract:Dendrolimus punctatus cytoplasmic polyhedrosis virus(DpCPV)genome dsRNA wag
extracted an d S4 was separated.RT—PCR amplification technique Wag adopted to clone the viral
genomic dsRNA S4 flor sequence determination.The results showed:the full length of S4 is 3262bp,it
contains one large open reading fram e an d encodes a polype ptide with 1058 amino acids in length.Tlle
similarity of predicted protein were 94% 、92% an d 22% with LdCPV、BmCPV an d RRSV respectively.
Amino acid sequences simi lar to a GTPase、methyltran sferase of Methanosarcina mazef Goel motifs
were also present in the deduced protein.
  Published online: 18 Jun 2003
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