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Virologica Sinica, 18 (3) : 254-258, 2003
Research Article
Construction and Toxicity Analysis of a Recombinant Viru s Contained Double Copies of v-cath Gene
College of Life Science,Central China Normal University,Wuhan 430079,China
2.Institute of Virology Wuhan University,Wuhan 43007 China
Abstract:A recombinant virus containing double copies of V—cath gene was constructed by using
Han pvid Bac—to—Bac system.ThiS recombinan t virus was named as dciHaNP、 because it was inserted
an other copy of V-cath gene controlled by iel promoter.Dot blot an d Northern blot an alysis showed that
this recombinan t virus was correct.The toxicity was measured by using Hz—AM l cells infec ted with the
recombinan t virus.Th e TCID5n ofdciHaNPlV is 3.16× l0 TCID5o/mL.
  Published online: 18 Jun 2003
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