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Virologica Sinica, 18 (3) : 275-278, 2003
Contents
Expression and Genic Imm unization of Glycoprotein gB Gene ofM arek’s disease virus
Key Laboratory of Animal Diseases Diagnostic and Immunology of Agriculture Ministry of China,Nanjing Agricultural University,Nanjing 210095.China
2.Department of Veterinary medicine,Foshan Science&Technology College,Nanhai 528231,Chinaf1.Key Laboratory of Animal Diseases Diagnostic and Immunology of Agriculture Ministry of China,Nanjing Agricultural University,Nanjing 210095.China
2.Department of Veterinary medicine,Foshan Science&Technology College,Nanhai 528231,China
 Correspondence:
(182.84KB)  
Abstract
gB gene of Marek’s disease virus (MDV) was inserted into pcDNA3, forming eukaryotic expression recombinant plasmid pcDNA3-gB. Recombinant plasmid pcDNA3-gB was identified by BamHⅠI and PCR. PcDNA3-gB was transfected into COS-7 cells in vitro. Expressed protein was detected by indirect immunofluorescence assay. Immune test groups were inoculated with pcDNA3-gB intramuscularly. Two weeks later, test groups were boosted with the same pcDNA3-gB. Since then, two weeks later chickens were challenged with vMDV. Immune effects showed that the immune protection rate of test group reached to 72.2%. The results proved that pcDNA3-gB DNA immunization elicited immune protection.
  Published online: 18 Jun 2003
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