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Virologica Sinica, 18 (3) : 288-291, 2003
Contents
Examination ofSpherical Viruses from Mytilus edulis
Key Laboratory of Mariculture Ecology,Dalian Fisheries College,Dalian 116024,China
 Correspondence:
(214.83KB)  
Abstract
Abstract:A lot of spherical virions were observed in internal organiT.ation such as gill and mantellum
of the diseased Mytilus edulis with negative staining method.The diameter of V on is around 8()_一
200nm with dual unit membran e.Sam e virions were observed in connective tissuese of infected organ s
with ultrathin section electronmicroscopic observ ation.Th e“occlusion body’’were observed in cell
matrix an d no inclusion bod y was present.A few same virions were observed in internal organization of
normal Ruditapes variega~,Neptunea cumingi an d Dstrea lalienwhanensis with negative staining
method.,nle normal Chlorostoma rusticum Can be infected by organizational liquid with the viruses
from diseased Mytilus edulis through mouth and injured section on skin.Th e virus was purified from the
diseased tissue by sucrose gradient centrifugation with 25 0oo r/min.3h.Th e virus sediment lies
between 2O% ~ 3O% of sucrose.
Key word:Mytilus edulis;SphericalAbstract:A lot of spherical virions were observed in internal organiT.ation such as gill and mantellum
of the diseased Mytilus edulis with negative staining method.The diameter of V on is around 8()_一
200nm with dual unit membran e.Sam e virions were observed in connective tissuese of infected organ s
with ultrathin section electronmicroscopic observ ation.Th e“occlusion body’’were observed in cell
matrix an d no inclusion bod y was present.A few same virions were observed in internal organization of
normal Ruditapes variega~,Neptunea cumingi an d Dstrea lalienwhanensis with negative staining
method.,nle normal Chlorostoma rusticum Can be infected by organizational liquid with the viruses
from diseased Mytilus edulis through mouth and injured section on skin.Th e virus was purified from the
diseased tissue by sucrose gradient centrifugation with 25 0oo r/min.3h.Th e virus sediment lies
between 2O% ~ 3O% of sucrose.
  Published online: 18 Jun 2003
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