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Virologica Sinica, 18 (4) : 387-390, 2003
Brief Reports
Plaque Electronic Im age Segm ent and Automatic Counting by Computer
1.College of Life Sciences,Wuhan University,Wuhan 430072,China
2.Department of Computer and Technology Huazhong University Science and Technology,Wuhan 430074,China
 Correspondence:
(167.55KB)  
Abstract
Abstrat:Plaques counting is a routine method in the theory research and practiced application of
microbiology.Because of weak contrast between plaque an d the background of the plate,an d the
plaques linking together,a serious error was produced using automatic counting by computer.So that
plaques have been counted by artificial method.In the present study,a new method was established for
automatic counting plaques by computer.At first,The plaques were formed by infecting Escherichia
coli with 入bacteriophage,and prepared as the method of soft—agar layer plate.Th e electrical images
counted the plaques were obtained by screening the plaques plates.A representative area,in which the
plaques were linked each other,was picked out from each image,an d han ded with watershed algorithm.
Th e plaques linking together were separated into individual plaque alone in the images.And then,the
images were counted with the method of growth algorithm based on area by computer.Th e exact effect
of counting plaques was in agreement with artificial method .It indicates the new method of counting
plaques can be used in automatic counting by computer.
  Published online: 5 Aug 2003
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