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Virologica Sinica, 18 (4) : 326-329, 2003
Research Article
HCV Core Protein Induces Apoptosis in Cos-7 Cell Line
Institute of virology,Wuhan University,Wuhan 430072,China
Abstract:Recombinant plasmid pCDNA3一Core was constructed by inserting Hepatitis C virus(HCV一1 1
core gene into the site between EcoR I and Xba I of eukaryotic expression vector pCDNA3.The
recombinan t plasmid pCDNA3一Core was tran sfected to COS一7 cellline by LipoVec .Expression of
core protein was detected by immunology Dot blot 24h after transfection.Th e nuclear cracks in
tran sfected cell was observed by Hoechst 3325 8 staining and the 1 80—200bp DNA ladders alSO was
detected 72h after transfection.Th ese results reveal that HCV Core protein Can induce apoptosis of
COS一7 cell and its function nlay play an importan t role in HCV chronic and persistan t infection.
  Published online: 5 Aug 2003
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