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Virologica Sinica, 18 (4) : 344-347, 2003
Research Article
Expression of Recom binant pEGFP·HPV16 E7 in Eukaryocyte
Virus Research Institution ofMedical College,Wuhan University Wuhan 430071,China
Abstract:In order to make bases for construction of animal tumor model which expresses EGFP—
HPV l 6E7,recombinant expression plasmid was constructed by techniques of gene recombination after
screening an d identifing by restriction and PCR.Then the recombinant was tran sfected into mouse liver
cancer cell by techniques of gene transfection and detected expression by fluoroscopy.pEGFP—HPV 1 6
E7 was identified by enzyme digestion an d PCR.Th e resent showed that the length .inserted location
and direction of the target gene was correct an d the expression of EGFP in tran sfected cell was observed
Because of it’S pEGFP—HPV 1 6E7 expression plasmid that mak es it easy to assess the expression of
EGFP-HPV 1 6E7 fusion protein and to sift the transfected cells.
  Published online: 5 Aug 2003
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