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Virologica Sinica, 18 (4) : 352-356, 2003
Research Article
M olecular Cloning and Sequence Analysis of Dendrolimus punctatus cytoplasmic polyhedrosis virus Segment 5
InstituteofVirology,Wuhan Univercity,Wuhan430072,China
 Correspondence:
(186.99KB)  
Abstract
Abstract:DendrolimUS punctatus polyhedra were purified from infected Dendrolimus punctatus Larvae
using density centrifugation.Dendrolimus punctatus cytoplasmic polyhedrosis viruses(DpCPV)were
subsequently extracted from polyhedra by SDS treatment,followed by l% agrose gel electrophoresis,
and the segement dsRNA 5 was purified after being excised from the ge1.On the basis of nucleotides
homology,five pairs of primers were designed.After the RT—PCR,five sub—clone segments were
obtained,following sequencing and splicing,the complete nucleotide seq uence of the genome segment
5(S5)of DpCPV was determined.The 285 1-nucleotide sequence contains a single long open reading
frame which span s nucleotides 7 1 to 27 1 4 an d was predicted to encode a protein with a molecular mass
of about 100.3kDa.Sequence an alysis an d deduced amino acid seq uence of DpCPV S5 revealed high
homology with proteins from LdCPV一1 an d BmCPV.From the phylogenetic tree,we can an alyse the
classification an d evolutionary relationship in viruses.
  Published online: 5 Aug 2003
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