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Virologica Sinica, 18 (4) : 367-370, 2003
Research Article
Construction of Eukarytic Expression Vector Containing PCV-2 ORF2 and Its Expression
College ofVeterinary Medicine,Nanjing Agriculture Universityt Nanfing 210095,China
 Correspondence:
(181.34KB)  
Abstract
According to the published OI 2 gene sequence of PCV一2.two primers were designed to
amplify the ORF2 gene from PK一1 5 cells inoculated by PCV一2.It was cloned into the pSecTag2
vector,and the recombinant plasmid named pSecORF2 was obtained.Another primer was designed to
amplify OI 2 gene including signal peptide sequence from pSecORF2.Then it was cloned into
pIREShyg vector.Th e recombinan t expression vector plRESiORF2 was constructed.CHO cells were
tran sfected in vitro with them. Th e expression of ORF2 gene was detected by indirect
immunofluorescence assay(If1A、.Further research iS underway to study the biological activity of OI
protein an d to establish the diagnostic kit of PCV
  Published online: 5 Aug 2003
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