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Virologica Sinica, 18 (4) : 391-393, 2003
Brief Reports
A Modified Technique for Purifying White spot syndrome virus
Key laboratory of Molecular Virology,Joint·Laboratory of Invertebrate Hrology,Wuhan Institute of Hrology,Chinese Academy of Sciences,Wuhan 430071,China
Abstract:The envelope proteins of White spot syndrome virus(WSSV)are very fragile and easy to be
destroyed during purification.It was difi cult to obtain a large quan tity of intact virions by routine
sucrose gradient centrifugation.After modifying the sucrose gradient by adding citrate sodium ,we can
obtain a large quantity of intact virions and nucleocapsids.Th is purified virions and nucleocapsids were
subsequently used for an alyzing viral structural proteins an d DNA extraction.Th e result showed that
this modified technique is very efi cient for virus purification.
  Published online: 5 Aug 2003
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