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Virologica Sinica, 18 (4) : 397-400, 2003
Brief Reports
A Backflushing Ultrafiltration Technique for Concentrating Cyanophage
College ofLife Science,Central China Normal University,Wuhan 430079,China
2.Joint-laboratory ofInvertebrate Virology,Wuhan Institute ofvirology,Chinese Academy ofSciences.Wh n 430071,China
Abastract:Most of the cyanophages were adsorbed onto the ultrafilter membrance when pre—filtrated
water sample passed through the tangential flow ultrafiltration system.In order to recover much more
cyanophages,we used a technique named‘‘backflushing ultrafiltration”.The results indicated that when
the volume of the backflush liquid was raised step by step,the concentration multiple of the cyanophage
would gradually decrease,while the recovery rate of the cyanophage would gradually increase.In our
performance,the maximal concentration multiple an d the recovery rate of cyan ophage could reach up to
30 times an d more than 90% severally.Th is method was tested to be efective for concentrating
cyan ophage from large volume liquid sample.M oreover,the concertration multiple of the cyan ophage
could be elevated to nearly one—hundred times when two—step backflushing ultrafiltration was adopted.
  Published online: 5 Aug 2003
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