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Virologica Sinica, 18 (4) : 401-403, 2003
Brief Reports
Establishm ent of Polymerase Chain Reaction for Discrim ination of VaccineStrains of Canine parvovirus
Guangdong Laboratory Animals Monitoring Institute,Guangzhou 510260,China
2.College of life Sciences, Zhongshan University,Guangzhou 510275.china
 Correspondence:
(128.82KB)  
Abstract
Two pairs of PCR primers were designed according to the sequances of the vaccine strain and
virulent strain of CPV.Heminested PCR method was established.Result of the first PCR amplification
showed the same am plified products of 574bp length ,after the second PCR am plification,the viru lent
strain produced the length 364bp fragment,but the vaccine strain couldn’t produce that.The products of
PCR were examined by electrophoresis an d restriction enzyme digestion.Th e result showed the length
of the fragment an d enzyme sites were as the same as those designed.Th e PCR assay of CPV was
proved to be spec ific an d sensitive.It shows that this method may be used in discriminating the vaccine
strain an d viru lent strain of CPV or monitoring the vaccinated can ine in order to aviod disease and
finan ciallosing.
  Published online: 5 Aug 2003
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