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Virologica Sinica, 18 (5) : 415-419, 2003
Research Article
Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome
The Center of Diagnosis and Treatmentfor Infectious Diseases of Chinese PLA,Tangdu Hospital,Fourth Military Medical University,Xi'an710038.China
Peripheral blood mononuclear cells(PBMCs)were separated on Ficoll—Hypaque density
gradient centrifugation from patients with Hemorrhagic Fever with Renal Syndrome (HFRS)and
inf~ted by EB virus(EBV),in Order to establish immortalized B lymphoblastoid cell line(B—LCL).
B—LCLs were then infected with the recombinant vaccinia viruse harboring Hantaan virus fHTNV)S
gene.Expression of nucleocapsid protein was detected using indirect immunofluorescent assay(IFA).
The results showed th at imm ortalized B—LCLs were set up.after B lymphocytes were infected by EBV
an d cultured for 4 weeks.B lym phocytes tran sform ed by EBV becam e enlar ged an d further gath ered
together.The HTNV nucleocapsid protein can be efectively expressed in B—LCLs仃ansinfected with
recom—binant vaccinia viruse harboring HTNV-S gene.
  Published online: 5 Oct 2003
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