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Virologica Sinica, 18 (5) : 420-422, 2003
Research Article
Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome
The Center of Diagnosis and Treatmentfor Infectious Diseases of Chinese PLA,Tangdu Hospital,Fourth Military Medical University,Xi’an 710038.China
Abstract:In order to construct the eukaryotic expression vector of月“man immunodeficieacy virus 1
(HIV-I)core protein gene(pCI—neoGAG),gag gene was acquired from the plasmid pKSGAG by
restriction endonuclease digestion (Xba I ISal I)and ligated onto the eukaryotic expression vector
pCI.neo.The construction of pCI.neoGAG was confirmed by restriction endonuclease digestion(Xba I
ISal I)analysis and DNA sequencing.Th en the pCI-neoGAG was transfected into p8 1 5 cells by
lipofectine and the expressed product was detected by indirect immunofluorescence after G4 1 8 selection
for 4 weeks.The results showed that the core protein was expressed in p8 1 5 cells successfully.which
will be used for the further test of HIV.1 DNA vaccine.
  Published online: 5 Oct 2003
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