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Virologica Sinica, 18 (5) : 428-431, 2003
Research Article
Construction of Prokaryotic Expression Vectors Harboring HV S and S Partial Gene Segments and Identification of Expressed Nucleocapsid Protein
Department ofInfectious Diseases,Tangdu Hospital,Fourth Military Medical University,X an 710038,China
Abstract:To study the antigenicity of prokaryotic expressed products of Hantavirus(HV)S partial
gene(502—1326bp)an d obtain data for serotyping antigen,the prokaryotic expression vectors pRSET
A—S,pRSET A—S—C were constructed respectively.The recombinant expression plasmids were fans—
formed into BL2 I(DE3)pLySs.Th e expression product were analyzed by SDS—PAGE and Western—blot.
Th e results showed that the proteins were expressed at high level respectively an d could be recognized
by patients’(infected by HTNV)sera distinctly and peculiarly.Th e truncated NP(aa155 to 429)could be
considered as serotyping an tigen.
  Published online: 5 Oct 2003
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