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Virologica Sinica, 18 (5) : 432-436, 2003
Research Article
Structural Protein rp 7 Gene Sequence Analysis of Human Group B Rotavirus Strain W H-2
1.Wuhan Centers for diseases Prevention and Control,Wuhan 430022,China
2.Tongji Medical College of Huazhong UniversityofScienceandTechnology,Wuhan430022,China
Abstract:The VP7 gene(vp7)ofHuman group B rotavirus(GBRV)strain WH一2 was amplified and
cloned into the plasmid pUCm—T by reverse transcription polymerase chain reaction(RT-PCR).Positive
vp7 gene cloned plasmid pT_VP7 was sequenced and analyzed by software GeneBee,the nucleotide
sequence of WH一2 vp7 gene showed high identities(more than 9O%)to that of other human GBRV,with
low identities(1ess than 65%)to that of animal GBRV.The secondary structure of WH-2 vp7 mRNA
was constructed by software RNAstructure 3.7 an d RnaViz 2.0,18 stem—loop structures were found on it.
Th e polypeptide encoded by W H一2 vp7 gene contains 249 amino acids with a calculated molecular
weight of 28.4 kDa.The protein contains three potential N—linked glycosylation sites an d a hydrophobic
signal—liked sequence at its amino termina1.Comparied with other GBRV,WH一2 vp7 demonstrated 99%
an d 95% of am ino acids similarity with ADRV and CAL.1 respectively,only 5 1% witIl IDⅡ .Th ese
findings suggested that W H一2 and ADRV might originate from a common an cestral virus,district from
an imal W H一2 vp7 reported SO far.
  Published online: 5 Oct 2003
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