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Virologica Sinica, 18 (5) : 464-467, 2003
Research Article
Partial Characteristic Comparison Between Two Aquareovirus Isolates
I.Wuhan Institute of Virology,Chinese Academy of Sciences。Wuhan 430071.China:2.State Key Laboratory of Freshwater Ecology and Biotechnology,Institute ofHydrobiology,Chinese Academy ofSciences,Wuhan 430072,China
Aquareoviruses,which belong to a newly identified genus of the family Reoviridae,are
agents infecting all kind of aquatic animals.Grass carp reovirus(GCRV)was isolated from outbreak
grass carp fingerling in South China freshwater region,and Threadfin reovirus(1f1W )is a Singapore
isolate from acute diseased marine threadfin fingerling.In this study,the partial characterization
between GCRV and TFV were compared.It showed that two viruses Can cause CPE in CIK cell line,but
not all the same in the other compared fish cell lines.In addition.their RNA genome compo sitions ale
diferent by both electrophoresis and RT_PCR.It is interesting to point out that.based on serological
related Westem blot analysis.me antiserum against GCRV could cross detecting some of the proteins
found in TFV but not all of the proteins.It suggested they may share some similar epitopes.
  Published online: 5 Oct 2003
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