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Virologica Sinica, 18 (5) : 473-477, 2003
Research Article
Study on Immunity of Recombinant Vaccinia Viruses Containing env Gene of EIAV
Division ofResearch on Virology and Immunology,National Centerfor AIDS/STD Control and Prevention.CDC, Peking 100050,China
2.Division ofResearch on EIAV,Harbin Veterinary Research Institute,Harbin 150001,China
In order to develop new vaccine candidate of Equine infectious anema virus(EIAV),env gene
of EIAV Chinese donkey leukocyte attenuated strain and its parental virus strain was inserted into the
downstream of pE/L promoter of pSC65 vector,separately.The resulted recombinant vaccinia viruses
were screened out by homologous recombination in TK region and the positive clone was confirmed
with blue plaque assay.Protein expression was examined by W estern blot.It was shown that the
recombinant vaccinia viruses successfully expressed the complete EIAV Env proteins an d induced
significan t cellular an d humoral immune responses in the Balb/c mi ce by intramuscular inoculation.
CTL specific lysis is 28% at most.Th e study will provide importan t elements for development of new
EIAV genetic engineering vaccine.
  Published online: 5 Oct 2003
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