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Virologica Sinica, 18 (5) : 482-485, 2003
Research Article
Expression of HaSNPV sod gene in E.coli
Institute of Applied Entomology,Zhejiang University,Hangzhou 3 1 0029,China
Abstract:The coding region of superoxide dismutase was amplified by using PCR method from
Helicoverpa armigera single enveloped nucleocapsid nucleopolyhedrovirus(HaSNPV)C 1 genome.The
PCR product was cloned into pGEM —T-easy vector and sequenced.Th e cloned coding region of
HaSNPV SOD was inserted into the expression vector pET blue一2 to form the recombinant plasmid
pETblue2/HaSNPV SOD and was then transformed into E.coli DE3 for expression.The SDS—PAGE
an alysis revealed that the expressed recombinan t SOD accumulated up to 37% of the total bacterial
protein.The enzyme activity of HaSNPV SOD was assayed by Pyrogallol autooxidation method.The
result showed that the revised enzyme activity of the expressed product in the total soluble protein was
694 U/mg.
  Published online: 5 Oct 2003
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