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Virologica Sinica, 18 (5) : 496-499, 2003
Research Article
Expression of Non—structural Protein S6 of Rice dwarf virus in E coil and Preparation of Its Antiserum
Department of Molecular Virologogy,Institue ofMicrobiology,Chinese Academy of Sciences,Beijing 100080,China
Abstract: gene of a Chinese isolate of Rice dwarf virus fRDV)was amplified by RT.PCR and then
cloned into pGEM—Teasy.Sequence analysis showed that its nucleotide sequence had high identity to a
Japan ese isolate of RDV an d contmned high percent of rare codons. l6 gene was expressed m inclusion
body with high yield in E.coli after being subcloned into expression vector pGEX一6P—1.Immune rabbit
witll S6 protein obtained the antiserum.The an tiserum having a titer of l:3000 tested by ELISA Can
specifically react wim the expressed S6 protein.Our study confn-med that the an tiserum could be used
to detect S6 protein in RDV-infected rice tissues.
  Published online: 5 Oct 2003
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