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Virologica Sinica, 18 (5) : 500-502, 2003
Research Article
Expression Analysis of Envelope Glycoprotein E2 Protein of Rubella virus JR23 Strain
Department of Virology,School of Public Health,Shandong University,Jinan 250012,China
To construct the recombinant plasmid containing envelope glycoprotein E2 gene of RV JR23
strain,to express E2 protein in BHl(2 l cells,and to analyze the antigenic sites of E2 protein of RV JR23
strain.E2 gene of RV JR23 strain was amplified bv I PCR.The PCR product was cloned into the
expression vector pBluescript II S and transformed into E.coli JM 109.an d the recombinant
plasmid was transfected into BHK21 cells with recombinant vaccinia viru s VTF一7.The expression was
detected by immunohistochemistry an d indirect immunofluorescence.The recombinan t plasmid
contained glycoprotein E2 gene.and expressed well in BHK2 l cells.The expressed E2 protein mainly
accumulated in plasma near the nuclei.Th ere are immunological epitopes of E2 protein on RV JR23
virions.The results showed that RV JR23 E2 transient expression system was successfully constructed.
This study ofers the basis for investigation of the relationship between the structure an d fu nction of RV
proteins,and interaction of viru s an d host cells.
  Published online: 5 Oct 2003
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