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Virologica Sinica, 18 (6) : 518-518, 2003
Research Article
Targeting the 5 untranslated Region ofHepatitis C virus by Intracellularly Expressed Small Interfering RNA
Modern VirologyResearchCenter,College ofLifeSciences,Wuhan University,Wuhan430072,China
Abstract:In this study,we inserted the 5 untranslated region(UTR)of Hepatitis C virus(HCV)
genome into the upstream of the reporter genes of enhanced green fluorescent protein (eGFP)and
luciferase.an d we also constructed the expression vector that Can express the short interfering RNAs
(siRNA) against the HCV 5 .UTR.The 5 .UTR.eGFP/luciferase an d the siRNA-prod ucing plasmid
were cotran sfected into Hela cells,an d the inhibition efect was detected by the intensity of the
fluorescence an d luminescence.Th e vesults showed that the light from the cotransfected cells was
obviously weaker than the negative control both in quality an d in quan tities,while density of the
cotran sfected cells had no diference with the control plasmid as detected by nucleus staining.TlliS work
demonstratedthat certain siRNA Can targetthe 5 UTR ofHCV whilenotoxic efecthadbeenobserved
in the cells.TlliS work is the basis for future research in which RNAi activity is supposed to be utilized
in the gene therapy with the the HCV infection.The siRNA is expressed intracellularly by vectors
instead of chemical synthesis,an d a new method Can be used as a model to quickly an d safely screen
effcctive siI AstargetingHCV
  Published online: 25 Dec 2003
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