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Virologica Sinica, 18 (6) : 538-540, 2003
Research Article
Construction of an Eukaryotic Vector Containing the Full-length HCV cDNA and Its Expression in M ammalian Cell
Department ofInfectious Diseases,Wuhan General Hospital,Guangzhou Military Command,Wuhan 430070,China
2.AcademyofMilitaryMedicalSciences,Beifing 100850,China
An eukaryotic vector containing the full—length Hepatitis C virus(HCV)gene,pCi—HCV,was
successfully constructed in this study.After~ansfected into HepG2 cells.it Was revealed to expleSS
HCV C and NS3 proteins in the cloned cells by immunofluorescence and immunohistochemistry as Say
respectively.It could be used for the experiments of the construction of HCV transgenic cellular model
and the further study on the replication of HCV through cDNA.
  Published online: 25 Dec 2003
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