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Virologica Sinica, 18 (6) : 553-556, 2003
Research Article
Isolation and Identification of Swine hlfIuenza Virus
Animal Virus Lab, Animal Science and Veterinary College,HuazhongAgricultural University,Wuhan4307~ China
 Correspondence:
(224.71KB)  
Abstract
Abstract:Three H3 subtype strains of Swine influenza virus(SlV),which were named SSH1,SFJ1 and
SHB1,were isolated from Shanghai,Fujian and Hubei.These isolates could grow and replicate in
chicken emb~o,and the hemagglutinin(HA)values were 1:2 ,1:2 and 1:2 ,respectively.The
hemagglutinin inhibitor(HI)titers of H subtype antiserum were 1:2 ,1:2 and 1:2。,respectively,
whereas they were negative in HI assays with Newcastle disease an tiserum.Under electron micrograph,
the virions showed various shapes.The mice infected witl1 allantoic fluid could arise syndromes an d
pathology;SIV could be isolated again and amplified by RT-PCR from the lung.RNA Was extracted
from SIV which Was purified from infected allantoic fluid,an d expected ban d Was observed via
RT—PCR.
  Published online: 25 Dec 2003
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