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Virologica Sinica, 18 (6) : 581-586, 2003
Research Article
Nucleotide Sequence Analysis and Prokaryotic Expression of S7 cDNA from Dendrolimus punctatus CPV Hunan Strain
The Key Laboratory of Molecular Virology,Wuhan Institute of Virology.Chinese Academy of Sciences,Wuhan 430071,China
2.Department ofSericulture and Silk shion,Anhui Agricultural University,Hefei 230036,China
Abstract:The nucleotide sequence of genome S7 cDNA (AY 1 80908) from Dendrolimus punctatus
cypovirus l Hunan strain(DpCPV—HN )was determined.S7 cDNA consists of 1 501 nucleotides and
encodes a protein of 448 amino acids with a molecular mass of 49.8 kDa.Th e 5 .an d 3 .terminus
conserved sequences are 5 一AGTAA一3 and 5 一GTTAGCC一3 。respectively. en compared wim S7
an d its putative protein of Lyman tria dispar cypovirus l an d Bombyx moil cypovirus 1.97.2% an d
87.0% identities in the nucleotide leve1.and 98.7% an d 92.8% identities in the amino acid level were
found。repectively.BLAST search program revealed some similarity between DpCPV·HN P50 an d
DnaK·like protein of Mycoplasma hominis.The cDNA fragment encoding DpCPV—HN P50 C259 was
also cloned an d inserted into pET.28a expressive vector after being digested with BamH I an d日 d m
to construct pET一28a/$7(59 1.1 37 1)recombinant expression vector.Expression of the fusion protein
was induced by IPTG in E.col BL21.Th e results indicated that the fusion protein with a molecular
mass of 37.3 kDa containing P50C259 was expressed abundan tly,and the fusion protein had an optional
level when induced by 1.0mmol/L IPTG over about 2 hours.
  Published online: 25 Dec 2003
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