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Virologica Sinica, 18 (6) : 587-592, 2003
Research Article
Helicoverpa armigera Nucleopolyhedrovirus Triggered Se-UCR Apoptosis is Blocked by Spodoptera exigua Nucleopolyhedrovirus
1.KeyLab MokcuI口r vimlogy.WuhanInstituteofvimlogy.ChineseAcademyofSciences,Wuhan430071.China
2.Lab of vimlogy.Wageningen University.Binnenhaven 11。6709PD Wageningen,the Netherlands
Abstract:Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus(HaSNPV)and Spodoptera
exigua muUicapsid nucleopolyhedrovirus(SeMNPV)are commercially produced as pesticides.As
pe sticides,both viruses have very narrow host range and Can not make cross infection.Trying tO
generate a rec ombinant baculovirus with broader host range by CO—infection and co-transfection,we
found that HaSNPV could trigger apoptosis of a exigua cell line Se·UCR.which could be blocked by
SeM NPV,but apoptosis did not occur in Se一30 1,a cell line also from exigua.W hen infected Se-UCR
with 5M OI,theapoptoticbodywasIn’stfound at 12hpi,obvious apoptosis couldbe observedat 24hpi,
at the end almost all ceHs were dead at 72 hpi.Those observations were confu-med by genome DNA
fragmen-ration analysis.Dot blot an alysis indicated that SeMNPV could also help HaSNPV genome
DNA an d then virus repficafion in Se-UCR cells,but not in Se-301.
  Published online: 25 Dec 2003
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