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Virologica Sinica, 18 (6) : 597-602, 2003
Research Article
Prokaryotic Expression of Three bro Genes from Helicoverpa armigera Singlenucleocapsid Nucleopolyhedrovirus and Generation of Antibodies
Joint-lab ofInvertebrate Virology and Key Laboratory of Molecular Virology,Wuhan Institute of Virology,Chinese AcademyofSciences,Wuhan430071,China
Abstract:Three baculovirus repeated open reading frame(bro)genes were amplified by PCR from the
genome DNA of HaSNPV.The PCR products were cloned into pBluescript KS(+)plasmid.The genes
were introd uced into the expression vector pProExHTb.After IFFG induction.the E coli DH5a strain
containing each of the recombinant plasmids expressed proteins with molecular weights of 32 kDa、64
kDa an d 58 kDa,respectively,which were in agreement with the prospeetation.Th e purified
recombinant proteins were used to immune the rabbits separately.Western blot an alysis using the
multiclonal an tibodies derived from the rabbits indicated that these an tibodies could react specifically
with thetargetproteinsan dwere suitableto beusedforfurtherfunctional an alysisofthebrogenes.
  Published online: 25 Dec 2003
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