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Virologica Sinica, 18 (6) : 607-610, 2003
Research Article
Harpin Expression and Induction of Bioactivity to Anti-TMV Infection
Instiute of Virology,Wuhan University,Wuhan 430072,China
Abstract:Harpin gene A,B,C and D fragments of Erwinia amylovora(Ea)were synthesized.The full
length Harpin gene was constructed by ligation,and then cloned into pET-23(+).Th e fusion Harpin
protein Was expressed in E.coli BL2 1,purified by His Bind Resin Ni”chromatography and subjected to
SDS—PAGE an d Western blot.The Mr of the Harpin was 45 kDa.5pL 301ag/mE Harpin induced
hypersensitive response on tobacco or capsicum leaves by using punctures or injection along nervation
in laminae respectively.Tobacco an d capsicum were treated with 30pg/mE Harpin protein by spraying
over the plant,and then Tobacco mosaic virus(TMV)was inoculated in the plants by mechanical
inoc ulation after 5 days.Th e TM V concentration in leaves was determined by ELISA.TM V infection in
tobacco an d capsicum treated with Harpin was repressed to a certain extent.
  Published online: 25 Dec 2003
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