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Virologica Sinica, 18 (6) : 614-615, 2003
Brief Reports
Rapid M olecular Diagnosis of Concurrent Infection of Porcine Reproductive and Respiratory Syndrome an d Pseudorabies
1.KeyLaboratoryofAnimalDiseaseDiagnosisandImmunologyNanjingAgricultural University,Nanjing 210095. China
2.Department ofAnimal Science.Agricultural College ofNingxia University.Yinchuan 750105.China 3 Animal Quarantine Institute of Agriculture Ministry,Qingdao 266032,China
Abstract:According to the nucleotide sequences of Porcine Reproductive and Respiratory Synd rome
V/ms VR2332 strain and Pseudorabies virus shan ghai strain provided in GenBank, two pairs of
primersweredesignedto amplifytheN geneofPRRSV byRT—PCR an dTkgeneofPRV byPCR. As
a result, a rapid molecular diagn osis with high specificity an d accuracy was set up.The result
indicated that PRRSV Was detected from 11 out of 33 samples, PRV was detected from 9 out of 33
samples, and CO—infec tion by PRRSV an d PRV was confirmed in 8 samples, the rate of co-infection
Was at24.2% .
  Published online: 25 Dec 2003
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