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Virologica Sinica, 19 (1) : 6-9, 2004
Research Article
Immunological Characteristics of a 27kDa Protein in the Bovine Sera
Department of Immunology and Key Laboratory of Molecular Medicine of Ministry of Education, Shanghai Medical College, Fudan University
Center for Gene Immunization and Vaccine Research, Shanghai 200032, China
 Correspondence:
(239.41KB)  
Abstract
To investigate the characteristics of an HBsAg-like protein in the bovine serum, SDS-PAGE and Western Blot were applied for identification of the molecular weight of this protein. A specific band with the molecular weight of 27kDa was detected in SDS-PAGE and Western Blot analyses. BALB/c mice were then immunized subcutaneously by using the 27kDa protein isolated by SDS-PAGE from bovine sera. Antibodies specifically reacted with HBsAg produced by HBV was detected in the serum derived from the mice immunized with the 27kDa protein. Thus, a 27kDa protein with antigenicity similar to HBsAg produced by HBV was found and characterized immunologically in bovine sera.
  Published online: 20 Feb 2004
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