Hello guest, Register  |  Sign In
Virologica Sinica, 19 (1) : 18-21, 2004
Research Article
Cloning and Sequence Analysis of N gene encoding Akabane virus Nucleotide Capsid Protein
1. Technology Center of Yunnan Entry-Exit Inspection and Quarantine, Kunming 650228, China
2. The key Opening Laboratory of Tropical and Subtropical Animal Virology of Ministry of Agriculture, Kunming 650224, China
The nucleocapsid protein (N) gene of Akabane virus strain YN isolated from bovine was amplified by RT-PCR. The product of 696 bp fragment was obtained as expected. The amplified fragment was then cloned into the pDM18-T vector and confirmed by PCR, endonuclease analysis, and sequencing. The results demonstrated that nucleotide homology with other published AKAV N genes was between 94.2%~98.3%, and the deduced amino acid homology was between 97.6%~99.5%, respectively. Therefore, nucleotide capsid protein gene of AKAV was conserved. Phylogenetic tree constructed with 17 reference strains of 696 bp of the N gene showed that AKAV-YN strain is related to Tinaroo strain.
  Published online: 20 Feb 2004
Home |  Resources |  About Us |  Support |  Contact Us |  Privacy |  Powered by CanSpeed | 
Copyright © 2015 Virologica Sinica. All rights reserved