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Virologica Sinica, 19 (1) : 18-21, 2004
Research Article
Cloning and Sequence Analysis of N gene encoding Akabane virus Nucleotide Capsid Protein
1. Technology Center of Yunnan Entry-Exit Inspection and Quarantine, Kunming 650228, China
2. The key Opening Laboratory of Tropical and Subtropical Animal Virology of Ministry of Agriculture, Kunming 650224, China
 Correspondence:
(179.44KB)  
Abstract
The nucleocapsid protein (N) gene of Akabane virus strain YN isolated from bovine was amplified by RT-PCR. The product of 696 bp fragment was obtained as expected. The amplified fragment was then cloned into the pDM18-T vector and confirmed by PCR, endonuclease analysis, and sequencing. The results demonstrated that nucleotide homology with other published AKAV N genes was between 94.2%~98.3%, and the deduced amino acid homology was between 97.6%~99.5%, respectively. Therefore, nucleotide capsid protein gene of AKAV was conserved. Phylogenetic tree constructed with 17 reference strains of 696 bp of the N gene showed that AKAV-YN strain is related to Tinaroo strain.
  Published online: 20 Feb 2004
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