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Virologica Sinica, 19 (1) : 49-53, 2004
Research Article
Interaction between Ubiquitin and IAP2 or IAP3 of Spodptera exigua Nucleopolyhedrovirus
Key Laboratory of Molecular Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
By using the yeast two hybrid system, in vitro assay for interaction between ubiquitin and IAP2 or IAP3 encoded by Spodptera exigua nucleopolyhedrovirus(SeNPV)has been done. The putative positive clones only existed on the plate of the SD/-Leu/-Trp/X-α-GAL, which suggested that ubiquitin interacted with IAP2 or IAP3 weakly, and ubiquitin was transferred to IAP2 or IAP3 through ubiquitin-activating enzyme (E1) and ubiquitin-conjugating enzyme (E2) in yeast. IAPs encoded by SeNPV may be the ubiquitin ligase (E3).
  Published online: 20 Feb 2004
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