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Virologica Sinica, 19 (1) : 73-75, 2004
Brief Reports
Construction and Expression of Eukaryotic Expression vector containing Hantaan Virus S gene and CpG motif
1. Department of Microbiology & Parasitology, China Medical University, Shenyang 110001,China
2. The Municipal Hospital of Infectious Disease, Shenyang 110003, China
 Correspondence:
(136.60KB)  
Abstract
To improve the effect of the gene immunization against Hantaan virus, we constructed the eukaryotic expression vector pTARGET-hans(ISS) containing Hantaan Virus S gene coding region and CpG motif by cloning S gene segment with CpG motif into eukaryotic expression vector pTARGETTM. After conformed by enzyme analysis, the recombinant expression vector pTARGET-hans(ISS) was transferred into Vero-E6 cells by electroporation and the transient expression of Hantaan virus nucleocapsid protein was detected by indirect immunofluorescence assay(IFA). In some transferred Vero-E6, the green fluorescence was showed, thus we can conclude that the eukaryotic expression vector pTARGET-hans(ISS) was successfully constructed and expressed in vitro, which will lay a foundation for further animal vaccination.
  Published online: 20 Feb 2004
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