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Virologica Sinica, 19 (1) : 79-81, 2004
Brief Reports
Establishment of Diagnostic Assay for Hantavirus with Microarray Techniques
Nanjing Military Institute, Nanjing Command, Nanjing 210002, China
To establish a rapid, sensitive and specific diagnostic assay for Hantavirus with microarray techniques, specific primers and probes were designed according to the conservative and specific DNA sequence of 76-118 strain and R22 strain. The probes were spotted on glass slides to form microarrays. The Cy3-labled single stranded DNA fragments prepared by dissymmetical PCR were hybridized with the probes on the glass slides. The microarrays were scanned and analyzed with a scanner. The results showed that the DNA microarray could detect the different typed DNA of HTN and SEO with adequate specificity and sensitivity. The developed DNA microarray and techniques might be a very useful method for diagnosis and prevention, and could be widely applied in specific pathogens detection of infectious diseases such as hemorrhagic fever with renal syndrome.
  Published online: 20 Feb 2004
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