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Virologica Sinica, 19 (2) : 114-119, 2004
Research Article
Generation of Full-genome SHIV-KB9 Clone and Its Replication in Human and Monkey Peripheral Blood Mononuclear Cells*
1. College of Life Sciences, Nankai University, Tianjin 300071, China
2. National Center for AIDS/STD Control and Prevention, Beijing 100050, China
 Correspondence:
(199.80KB)  
Abstract
SHIV-KB9 chimeric virus was constructed by two constructs representing the 3’ and 5’ fragments of the viral genome. This full-genome SHIV-KB9, with large size and long terminal repeat sequence (LTR), could be maintained stable in E.coli JM109 strain in the cultivation condition of 30℃ and 180r/min. The infectivity of the full-length SHIV-KB9 in human peripheral blood mononuclear cells (PBMC), cynomolgus and rhesus monkey PBMC was characterized by polymerase chain reaction (PCR), reverse transcription PCR (RT-PCR) and Simian immunodeficiency virus (SIV) p27 gag antigen titration assay.
Key Words: SHIV-KB9;  Full-genome;  Infection;  PBMC
  Published online: 20 Apr 2004
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