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Virologica Sinica, 19 (2) : 129-132, 2004
Research Article
Regulation of RU5 Region to Gene Expression of BFV 3026
College of Life Sciences, Nankai University, Tianjin 300071, China
In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.
  Published online: 20 Apr 2004
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